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Detection and Sequencing of Norwalk‐Like Viruses from Stool Samples in Japan Using Reverse Transcription‐Polymerase Chain Reaction Amplification
Author(s) -
Nakayama Miyuki,
Ueda Yuhichi,
Kawamoto Hiroyoshi,
Hanjun Yue,
Saito Kunihiko,
Nishio Osamu,
Ushijima Hiroshi
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03343.x
Subject(s) - virology , polymerase chain reaction , norwalk virus , biology , reverse transcription polymerase chain reaction , outbreak , rotavirus , reverse transcriptase , virus , norovirus , gene , genetics , messenger rna
Norwalk‐like viruses were detected in Japan in 12% (26/209) of patients with nonbacteria and non‐rotavirus gastroenteritis in an outpatient clinic of a hospital from 1991 to 1994 by reverse transcription‐polymerase chain reaction. They were also present in 7% (26/378) of total samples including those from rotavirus‐positive gastroenteritis patients. In addition, the viruses were recovered in samples from 15 of 17 patients which were collected during outbreaks of gastroenteritis in various places in Japan by the same method. The DNA sequence of the polymerase region from patients at the hospital (sporadic cases) showed that two subgenogroups, similar to UK1‐6 in genogroup G1 and to UK1‐1 in genogroup G2 (Ando et al, J Clin Microbiol, 1995, 33: 64‐71) exist in Japan. The latter was more frequently found.