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Isolation and Characterization of Teichoic Acid‐Like Substance as an Adhesin of Staphylococcus aureus to HeLa Cells
Author(s) -
Matsuura Takashi,
Miyake Yoichiro,
Nakashima Seiji,
Komatsuzawa Hitoshi,
Akagawa Yasumasa,
Suginaka Hidekazu
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03341.x
Subject(s) - teichoic acid , hela , lipoteichoic acid , bacterial adhesin , coomassie brilliant blue , biology , staphylococcus aureus , biochemistry , antiserum , microbiology and biotechnology , cell envelope , cell wall , cell , bacteria , peptidoglycan , staining , antigen , immunology , escherichia coli , genetics , gene
A cell wall component that bound to HeLa cells (HeLa cell‐binding CWC) was isolated from a clinical isolate of Staphylococcus aureus . The HeLa cell‐binding CWC was resistant to heat (100 C, 1 hr) and proteases, did not stain with Coomassie Brilliant Blue R‐250 on SDS‐PAGE but stained as a broad band with antiserum against the strain on Western blots. These data suggest that the HeLa cell‐binding CWC is not a protein, and may be teichoic acid. Purified teichoic acid bound to HeLa cells, whereas fractions without teichoic acid did not. In Western blots, HeLa cell‐binding CWC appeared as a broad band of less than 35 kDa, similar to that of purified teichoic acid. These data suggest that the HeLa cell‐binding CWC obtained in this study is teichoic acid. Teichoic acid inhibited S. aureus adherence to HeLa cells and bound to the cells time and dose dependently, in a saturable and reversible manner, and therefore appears to be an adhesin of S. aureus to HeLa cells.