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Reactivation of Herpes Simplex Virus Type 1 in Cells of Latently Infected Mouse Trigeminal Ganglia Cultured in Serum‐Free Medium
Author(s) -
Kimura Susumu
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03332.x
Subject(s) - herpes simplex virus , virology , biology , trigeminal ganglion , virus , cell culture , simplexvirus , herpesviridae , viral disease , genetics , sensory system , neuroscience
Herpes simplex virus type 1 (HSV‐1) was reactivated more rapidly in cells of latently infected mouse trigeminal ganglia which were cultured in serum‐free medium (after 3.7 days of cultivation) than in those cultured in serum‐containing Dulbecco's modified Eagle's medium (after 8.5 days of cultivation). The concentration of calcium ion (Ca 2+ ) in the medium affected HSV‐1 reactivation in ganglionic cultures, and 0.9 mM was the optimum concentration for the reactivation. Reactivation was delayed significantly in ganglia put into culture 4 months or more after infection compared with those cultured 1 month after infection.