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Crystallization of Synthetic Escherichia coli ‐Type Lipid A
Author(s) -
Kato Nobuo,
Naito Setsuko,
Arakawa Yoshichika,
Sugiyama Tsuyoshi,
Ito Hideo,
Ohta Michio,
Sasaki Kyoyu
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03314.x
Subject(s) - crystallization , escherichia coli , crystallography , hexagonal crystal system , basal plane , hydrolysis , x ray crystallography , diffraction , chemistry , materials science , biochemistry , organic chemistry , physics , optics , gene
Synthetic Escherichia coli ‐type lipid A formed hexagonal plate crystals when it was precipitated by the addition of 2 volumes of 95% ethanol containing 375 mM MgCl 2 and incubated in 70% ethanol containing 250 mM MgCl 2 at 4 C for 10 days. Analyses of crystals by electron diffraction and synchrotron X‐ray diffraction showed that crystals consist of hexagonal lattices with the lattice constant (a side of the lozenge as a unit cell on the basal plane) of 4.62 Å and the longitudinal axis (perpendicular to the basal plane) of 49.3 ±1.3 Å. Results suggest that the previous finding that various kinds of R‐form lipopolysaccharides crystallized but free lipid A isolated by acid hydrolysis from Re lipopolysaccharide did not crystallize under the same experimental conditions (Kato et al, J. Bacteriol., 172: 1516‐1528, 1990) is due to structural changes of lipid A occurring during the procedure of isolation of free lipid A.