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A New Primer Pair for Detection of Chlamydia pneumoniae by Polymerase Chain Reaction
Author(s) -
Kubota Yoshifumi
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03313.x
Subject(s) - biology , polymerase chain reaction , chlamydiales , chlamydiaceae , chlamydia psittaci , primer (cosmetics) , chlamydia , chlamydophila pneumoniae , chlamydia trachomatis , microbiology and biotechnology , dna , virology , gene , genetics , chemistry , organic chemistry
In order to improve the detection and identification of Chlamydia pneumoniae , new primers for polymerase chain reaction (PCR) were designed based on the DNA base sequence within the 53‐kDa protein gene, which is specific for C. pneumoniae . The primers permitted the identification of 24 C. pneumoniae strains collected from different geographical locations, but no reaction was observed with C. trachomatis, C. psittaci nor C. pecorum . The primers were unable to amplify the DNA of bacteria commonly related to respiratory tract infections. The positive amplification was achieved with only 9 EBs/assay. Therefore, the new primers seem to be useful in the diagnosis of C. pneumoniae infections.