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Different Sensitivity to Streptolysin‐O of Cells in Macrophage Lineage
Author(s) -
Tanigawa Takahiko,
Suzuki Jun,
Ueta Tsuyoshi,
Katsumoto Tetsuo,
Tanaka Yoshinori
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03308.x
Subject(s) - streptolysin , biology , cytotoxic t cell , macrophage , cell culture , microbiology and biotechnology , inducer , lineage (genetic) , myeloid leukemia , immunology , in vitro , biochemistry , genetics , gene , bacterial protein
We have surveyed the sensitivity of cells in macrophage lineage to Streptolysin‐O (SLO). SLO had cytotoxic activity on immature myeloid cell lines such as M1 and WEHI‐3BD+. SLO was toxic to the cells after a 2‐hr incubation. However, mature macrophage cell lines such as A640‐BB‐2, J774, and P388D1 were not sensitive to the same dose of SLO. After M1 cells were treated with leukemia inhibitory factor (LIF), a differentiation‐inducer to macrophage, these cells became insensitive to SLO in one day. This cytotoxic action of SLO was inhibited by pretreatment with anti‐Streptolysin‐O antibody or cholesterol. These results indicate that SLO has different effects on macrophage lineage.