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Analysis of the Epitopes Recognized by Mouse Monoclonal Antibodies Directed to Yersinia enterocolitica Heat‐Shock Protein 60
Author(s) -
Yamaguchi Hiroyuki,
Miura Hitoshi,
Ohsumi Kazuoki,
Osaki Takako,
Taguchi Haruhiko,
Yamamoto Tomoko,
Hanawa Tomoko,
Ogata Sachio,
Kamiya Shigeru
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03307.x
Subject(s) - epitope , yersinia enterocolitica , hsp60 , monoclonal antibody , biology , amino acid , peptide sequence , heat shock protein , microbiology and biotechnology , antibody , homology (biology) , biochemistry , genetics , gene , bacteria , hsp70
To determine amino acid sequences of the epitopes recognized by monoclonal antibodies (mAbs) 3C8 and 5C3 directed against Yersinia enterocolitica heat‐shock protein (HSP60), a dot blot analysis was perfomed using synthesized peptides of Y. enterocolitica HSP60 such as peptides p316‐342, p327‐359, p340‐366, p316‐326, p316‐321, p319‐323, and p321‐326 which represent positions of amino acids in Y. enterocolitica HSP60. The dot blot analysis revealed that 5C3 mAb reacted with p316‐342, p316‐326 and p321‐326, and 3C8 mAb p316‐342 and p316‐326. These results indicate that the epitopes recognized by the mAbs were associated with eleven amino acids, Asp Leu Gly Gln Ala Lys Arg Val Val Ile Asn, of p316‐326. The sequence homology between p316‐326 of Y. enterocolitica HSP60 and the rest of the HSP60 family suggests that the five amino acids of Lys, Arg, Val, Ile and Asn, which are highly conserved in the HSP60 family, might be related with the epitope recognized by 3C8. In contrast, it was also demonstrated that three amino acids of Leu, Gly and Val, which are not well conserved in the HSP60 family, might be related to the epitope recognized by 5C3.