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Effects of Granulocyte‐Macrophage Colony Stimulating Factor (GM‐CSF) on Biomaterial‐Associated Staphylococcal Infection in Mice
Author(s) -
Rózalska Barbara,
Ljungh Asa,
PaziakDomańska Beata,
Rudnicka Wieslawa
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01162.x
Subject(s) - granulocyte macrophage colony stimulating factor , staphylococcus aureus , staphylococcus epidermidis , microbiology and biotechnology , staphylococcal infections , granulocyte colony stimulating factor , staphylococcus , immunology , granulocyte , biomaterial , peritoneum , biology , micrococcaceae , macrophage , bacteria , in vitro , medicine , antibacterial agent , cytokine , antibiotics , pathology , chemotherapy , biochemistry , genetics , biomedical engineering
Staphylococcal infections are a major complication in the usage of biomaterials. Different modifications of polymers have been made to reduce the incidence of such infections. We studied the effects of modifying heparinized polyethylene (H‐PE) with mouse recombinant granulocyte‐macrophage stimulating factor (rGM‐CSF). The elimination of staphylococci (Staphylococcus aureus, S. epidermidis) from the peritoneum of mice implanted with rGM‐CSF‐coated H‐PE was slightly more effective than the elimination of the bacteria from the peritoneum of animals implanted with uncoated H‐PE. Most interestingly, the number of staphylococci present in the biofilms covering rGM‐CSF‐coated implants were significantly lower than the number of bacteria detected on the surface of H‐PE not coated with rGM‐CSF. In vitro , rGM‐CSF restored the anti‐bacterial potency of the phagocytes, which had been reduced by surface contact with H‐PE. The results suggest that modification of biomaterials with rGM‐CSF could be one way of preventing staphylococcal infections; especially in neutropenic disorders, which constitute the highest risk factor for foreign body‐associated infections.