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Differential Expression of a Mycobacterium tuberculosis Protein Recognized by a Mouse Monoclonal Antibody
Author(s) -
Cummings Patrick J.,
Rowland Sharon S.,
Hooper Nancy E.,
Schwalbe Richard S.
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01155.x
Subject(s) - monoclonal antibody , mycobacterium tuberculosis , biology , western blot , microbiology and biotechnology , antibody , virology , monoclonal , cell culture , blot , tuberculosis , immunology , biochemistry , gene , genetics , medicine , pathology
Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme‐linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65 kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65 kDa Mtb protein that is over‐expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.