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Production, Binding Characteristics and Protective Immunity of Monoclonal Antibody to Pneumococcal Type‐9V Conjugate
Author(s) -
Lee ChiJen,
Karpas Arthur,
Wang Theresa R.,
Kosaka Tadashi,
Koizumi Kaio
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01151.x
Subject(s) - immunogen , monoclonal antibody , antiserum , antigen , biology , antibody , conjugate , microbiology and biotechnology , immunology , mathematical analysis , mathematics
A monoclonal antibody (MAb) to pneumococcal type‐9V polysaccharide (PS) was produced using PS conjugated to inactivated pneumolysin as the immunogen. The MAb to 9V PS was of the IgG 1 subclass. The antigen‐antibody reaction increased rapidly at low concentrations and reached a plateau at 10 μg PS/ml as measured by nephelometry of the group 9 PS against 9V MAb binding. In contrast, the binding of group 9 PS against rabbit 9V antiserum (AS) increased proportionally and continued to increase up to the highest concentration of PS tested (20 μg PS/ml). The 9V MAb reacted with all group 9 PSs (9A, 9L, 9N and 9V) by immunodiffusion. In the homologous 9V Ag‐MAb reaction, there were marked differences in the inhibition of binding by the cross‐reactive 9L PS (19.2% inhibition) and the 9N PS (0.2%). In contrast, inhibition of the homologous 9V Ag‐rabbit AS binding by cross‐reactive 9L and 9N PSs ranged from 57.8 to 62.7%. Removal of the O‐acetyl group from 9V PS by alkali hydrolysis resulted in decreased binding with rabbit 9V AS. However, the binding reaction with 9V MAb was less affected by the loss of O‐acetyl content. The 9V MAb was both opsonic and passively protected young mice against challenge with type‐9V pneumococci.