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Investigation of IFN Type‐I Receptor and IFN Regulatory Factor Expression Relating to Induction of 2′, 5′‐Oligoadenylate Synthetase in Cells Persistently Infected with the Mumps Virus
Author(s) -
Fujii Nobuhiro,
Yokosawa Noriko,
Ishida Setsuko,
Shirakawa Sachiko,
Kubota Toru,
Indoh Tomokazu,
Fujinaga Kei,
Yashiki Teruo
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01141.x
Subject(s) - biology , mumps virus , interferon , receptor , virology , virus , irf1 , gene expression , interferon regulatory factors , microbiology and biotechnology , interferon type i , transcription factor , gene , genetics
Poor induction of interferon‐induced 2′, 5′‐oligoadenylate synthetase (2–5AS) activity has been demonstrated in cells persistently infected with the mumps virus or human T‐lymphotropic virus type‐I (HTLV‐I). The suppression of 2–5AS induction is the result of the repression of 2–5AS gene expression at the transcription level. In a general way, after the binding of interferon‐α (IFN‐α) to cell surface‐specific receptors, expression of 2–5AS gene is thought to be regulated by some transacting factors, IFN‐regulatory factors (IRF‐1 and IRF‐2) and the IFN‐stimulated gene factor (ISGF‐3, a complex consisting of STAT‐1α, STAT‐2 and p48). To clarify the cause of the suppression mechanism(s), fluctuation in the number of IFN receptors and the levels of mRNAs in both IRF‐1 and IRF‐2 were examined in cells persistently infected with the mumps virus (FLMT and KBMT). There were few defferences in the number of IFN receptors and the level of IRF‐2 mRNA between persistently infected cells and uninfected control cells. After the treatment of cells with IFN, a slight reduction of IRF‐1 mRNA was found in persistently infected cells as compared with that of the uninfected control cells.