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Acid‐Fastness of Nocardia asteroides GUH‐2 Cultured in Brain Heart Infusion Broth Supplemented with Paraffin
Author(s) -
Kohbata Shunro
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01131.x
Subject(s) - brain heart infusion , nocardia , microbiology and biotechnology , biology , alcohol , bacteria , chromatography , chemistry , biochemistry , genetics , agar
Nocardia asteroides GUH‐2 was not acid‐fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth supplemented with paraffin, many filamentous cells showed acid‐fastness after treatment with 1% acid‐alcohol as the decolorizing agent. When treated with 3% acid‐alcohol, filamentous cells were not acid‐fast. In addition to the acid‐fast filamentous cells of nocardiae, unknown acid‐fast spherical bodies were observed in the paraffin‐supplemented BHI broth cultures.