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Effects of Nerve Growth Factor and Phorbol Derivative on Reactivation of Herpes Simplex Virus Type 1 in Cultured Cells of Latently Infected Adult Mouse Trigeminal Ganglia
Author(s) -
Kimura Susumu
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01122.x
Subject(s) - herpes simplex virus , nerve growth factor , biology , trigeminal ganglion , viral replication , virology , herpesviridae , virus , cell culture , alphaherpesvirinae , simplexvirus , ratón , phorbol , endocrinology , medicine , microbiology and biotechnology , viral disease , receptor , biochemistry , phosphorylation , genetics , protein kinase c , sensory system , neuroscience
Reactivation of herpes simplex virus type 1 (HSV‐1) occurred rapidly in cells of latently infected adult mouse trigeminal ganglia which were cultured in serum‐free medium in the presence of sufficient nerve growth factor (NGF). However, HSV‐1 reactivation was delayed significantly in ganglionic cultures in the absence of exogenous NGF or in cultures treated with 2‐aminopurine in the presence of NGF. The delayed viral reactivation in ganglionic cultures without NGF was accelerated by treatment with phorbol myristate acetate or dibutyryl cyclic AMP. Culture conditions which affected HSV‐1 reactivation did not affect replication of HSV‐1 in normal ganglionic cultures.