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Genetic Analysis of Borrelia burgdorferi Sensu Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination
Author(s) -
Kee SunHo,
Yoon JungHoon,
Oh HeeBok,
Park YongHa,
Kim YoonWon,
Cho MinKee,
Park KyungSeok,
Chang WooHyun
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01115.x
Subject(s) - sensu , borrelia afzelii , borrelia garinii , biology , borrelia burgdorferi , phylogenetic tree , borrelia , 16s ribosomal rna , restriction fragment length polymorphism , genetics , phylogenetics , microbiology and biotechnology , spirochaetaceae , sequence analysis , gene , polymerase chain reaction , zoology , antibody , genus
Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (Hin dIII and Eco RV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii . 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii ‐related group and some minor group such as B. garinii also existed.