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Involvement of Two Types of TNF Receptor in TNF‐α Induced Neutrophil Apoptosis
Author(s) -
Gon Songi,
Gatanaga Tetsuya,
Sendo Fujiro
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01095.x
Subject(s) - tumor necrosis factor alpha , apoptosis , biology , receptor , cycloheximide , microbiology and biotechnology , antibody , antiserum , immunology , biochemistry , protein biosynthesis
We previously demonstrated that tumor necrosis factor‐α (TNF‐α) induces rapid human neutrophil apoptosis. In this paper, we examined which of the TNF receptors, p55 kDa TNF receptor (55‐R) or p75 kDa TNF receptor (75‐R), or both are involved in this process using specific rabbit antisera. Antibodies to 55‐R (anti55‐R) or 75‐R (anti75‐R) alone did not induce neutrophil apoptosis. Further addition of cycloheximide and anti‐rabbit immunoglobulin to anti55‐R but not to anti75‐R accelerated apoptosis, although anti75‐R augmented the capacity of anti55‐R to do so. These results suggest that 55‐R is a prerequisite for TNF‐α induced neutrophil apoptosis.