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Crystallization of an R‐Form Lipopolysaccharide from Klebsiella pneumoniae
Author(s) -
Kato Nobuo,
Ohta Michio,
Arakawa Yoshichika,
Naito Setsuko,
Sugiyama Tsuyoshi,
Ito Hideo,
Kido Nobuo,
Sasaki Kyoyu,
Asai Junpei
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01087.x
Subject(s) - tris , klebsiella pneumoniae , crystallization , lipopolysaccharide , hexagonal crystal system , crystallography , polysaccharide , strain (injury) , biology , microbiology and biotechnology , escherichia coli , chemistry , biochemistry , anatomy , organic chemistry , gene , endocrinology
An R‐form lipopolysaccharide (LPS) from Klebsiella pneumoniae strain LEN‐111 (O3 – :K1 – ) formed crystals, whose shapes were elongated hexagonal plates, trapezoid plates, and rhomboid plates, and whose greatest dimensions were 3.1 × 0.8 μm, when it was suspended in 50 mM Tris buffer at pH 8.5 containing 5 mM MgCl 2 and kept at 4 C for as long as 870 days. K. pneumoniae LEN‐111 synthesized LPS molecules possessing incomplete repeating units of the O‐antigenic polysaccharide portion besides the R‐form LPS because of a leaky characteristic, but crystals consisted exclusively of the R‐form LPS. Although the size of crystals was not large enough for X‐ray analysis and limited crystallographic information was available, it was suggested that the crystals consist of hexagonal lattices with an a axis of 4.62 Å and c axis of 79.8 ±2.6 Å. The present results showed that R‐form LPS lacking the O‐antigenic polysaccharide portion tends to form crystals during long‐term incubation in Tris buffer at pH 8.5 containing MgCl 2 at 4 C.