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Sulfonamide Resistance Gene in a Transferable R Plasmid of Pasteurella piscicida
Author(s) -
Kim EunHeui,
Aoki Takashi
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb01085.x
Subject(s) - biology , plasmid , gene , genetics , nucleic acid sequence , coding region , restriction map , pasteurella , sequence analysis , sequence (biology) , microbiology and biotechnology , bacteria
Abstract The sulfonamide resistance (SA r ) determinant was cloned from a transferable R plasmid of Pasteurella piscicida , pSP9351, and the sequence was determined. The resistance gene (pp‐ sul ) was localized to an approximately 1‐kb region that includes the Pst I‐ Eco RI site in the restriction map. An open reading frame coding a sul II ‐type gene composed of 810 nucleotides was identified. A direct repeat sequence was shown in the 5′ flanking region of pp‐ sul , and a plasmid recombinational event may have occurred during the construction of pSP9351. In the 3′ flanking region of the gene, a sequence homologous to the 5′ noncoding sequence of the trimethoprim resistance gene, dhfr IX was found.