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Recognition of Nonstructural Protein Peptides by Cytotoxic T Lymphocytes Raised against Japanese Encephalitis Virus
Author(s) -
MuraliKrishna Kaja,
Ramireddy Bommireddy,
Ravi Vasanthapuram,
Manjunath Ramanathapuram
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03294.x
Subject(s) - ctl* , virology , cytotoxic t cell , japanese encephalitis , ns3 , virus , biology , polyclonal antibodies , peptide , encephalitis , antibody , microbiology and biotechnology , immunology , in vitro , biochemistry , hepatitis c virus
We have previously reported that Lyt2 + cytotoxic T lymphocytes (CTL) can be raised against Japanese encephalitis virus (JEV) in BALB/c mice. In order to confirm the presence of H‐2K d ‐restricted CTL and to examine their cross‐recognition of West Nile virus (WNV), we tested the capacity of anti‐JEV CTL to lyse uninfected syngeneic target cells that were pulsed with synthetic peptides. The sequence of the synthetic peptides was predicted based upon the H‐2K d binding consensus motif. We show here that preincubation of uninfected syngeneic targets (P388D1) with JEV NS1‐ and NS3‐derived peptides [NS1 (891‐899) and NS3 (1804‐1812)], but not with JEV NS5‐derived peptide [NS5 (3370‐3378)], partially sensitized them for lysis by polyclonal anti‐JEV CTL. These results indicate the CTL recognition of NS1‐ and NS3‐derived peptides of JEV.