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Flow Cytometric Analysis Using Lipophilic Dye PKH‐2 for Adhesion of Vibrio cholerae to Intestine 407 Cells
Author(s) -
Taguchi Haruhiko,
Osaki Takako,
Yamaguchi Hiroyuki,
Kamiya Shigeru
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03286.x
Subject(s) - vibrio cholerae , microbiology and biotechnology , biology , vibrionaceae , bacteria , genetics
A comparative study of indirect and direct flow cytometric analysis for adherence of Vibrio cholerae to Intestine 407 cells was performed. The direct flow cytometric analysis employed the lipophilic dye PKH‐2. It was concluded that direct flow cytometry using the lipophilic dye PKH‐2 is useful and convenient for analyzing bacterium‐host cell interactions, since it does not require any specific antibody as the first antibody.