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Expression of 18.6/CD23 Antigen on Human Lymphoid Progenitor Cell Lines and Phorbol 12‐Myristate 13‐Acetate (PMA)‐Induced Microglia‐Shaped Cells
Author(s) -
Okumura Akiou,
Kishi Hiroyuki,
Tagoh Hiromi,
Minowada Jun,
Muraguchi Atsushi
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03285.x
Subject(s) - biology , antigen , progenitor cell , microbiology and biotechnology , cell culture , cd23 , b cell , b 1 cell , antibody , immunology , t cell , antigen presenting cell , stem cell , immunoglobulin e , immune system , genetics
The nature of lymphoid progenitors and factor(s) determining commitment to either the T‐ or B‐lymphocyte pathway are poorly understood in the human system. In this study, we generated a monoclonal antibody (MoAb), 18.6, that recognizes a cell surface antigen on a human lymphoid progenitor cell line (FL4.4). MoAb 18.6 reacted with lymphoid progenitor lines, B lymphoid cell lines, and myelomonocytic cell lines. It did not react with any T cell or erythroid leukemic cell lines. Two color FACS analyses of normal lymphoid tissues showed that MoAb 18.6 reacted with a majority of CD20 + mature B cells and a minority of CD64 + monocytes. Molecules of 3 different sizes with MW of 34, 45, and 68 Kd were precipitated with MoAb 18.6 from the lymphoid progenitor cell line. The 18.6 antigen was not expressed on a fetal liver‐derived lymphoid progenitor‐like cell line, FL1.4, which has the capacity to differentiate into microglia‐shaped cells upon PMA‐stimulation. Stimulation of FL1.4 cells with PMA induced expression of the 18.6 antigen within 24 hr and the microglia‐shaped cells stained positively with MoAb 18.6. Finally, cloning of a cDNA that encoded the 18.6 antigen revealed that the 18.6 antigen is identical to the CD23 antigen. Taken together, these data suggest that the 18.6/CD23 antigen is expressed on lymphoid precursors at a very early stage of differentiation.