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Genetic Identification of Staphylococcus aureus by Polymerase Chain Reaction Using Single‐Base‐Pair Mismatch in 16S Ribosomal RNA Gene
Author(s) -
Saruta Katsutoshi,
Hoshina Sadayori,
Machida Katsuhiko
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03280.x
Subject(s) - staphylococcus aureus , biology , polymerase chain reaction , 16s ribosomal rna , ribosomal rna , microbiology and biotechnology , base pair , gene , ribosomal dna , dna , bacteria , genetics , phylogenetics
Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single‐base‐pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureus without requiring additional analytical techniques. Twenty‐eight staphylococcal and non‐staphylococcal strains were tested to verify the specificity of the assay, and only S. aureus strains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus .