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Analysis of the Relationship between Cellular Thymidine Kinase Activity and Virulence of Thymidine Kinase‐Negative Herpes Simplex Virus Types 1 and 2
Author(s) -
Suzutani Tatsuo,
Koyano Shin,
Takada Minoru,
Yoshida Itsuro,
Azuma Masanobu
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03271.x
Subject(s) - thymidine kinase , virulence , herpes simplex virus , biology , virology , thymidine , virus , strain (injury) , viral replication , kinase , microbiology and biotechnology , gene , dna , genetics , anatomy
The virulence of thymidine kinase‐negative herpes simplex virus type 1 (HSV‐1; VRTK − strain) and type 2 (HSV‐2; UWTK − strain) was studied in comparison with that of their parental strains (VR‐3 and UW‐268, respectively) in an encephalitis model of adult (4‐week‐old) and newborn (3‐day‐old) mice. Viral thymidine kinase (TK) activity was essential for the maximum expression of virulence of HSV‐1, because the 50% lethal dose (LD 50 ) of VRTK − was 60 times higher than that of VR‐3 in the brains of newborn mice expressing high levels of cellular TK activity. However, the UWTK − strain showed the same virulence as the parental strain in newborn mice, despite the lack virulence in adults, suggesting that replication of the UWTK − strain was completely supported by cellular TK activity. This difference in the role of viral and cellular TKs for virus growth between HSV‐1 and HSV‐2 was confirmed with the one‐step growth of virus strains in L‐M and L‐M(TK − ) cells.