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Construction of a Combined Not I/ Sma I Physical and Genetic Map of Moraxella ( Branhamella ) catarrhalis Strain ATCC25238
Author(s) -
Furihata Kakuya,
Sato Kaori,
Matsumoto Hideki
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03266.x
Subject(s) - biology , moraxella catarrhalis , moraxella (branhamella) catarrhalis , strain (injury) , pulsed field gel electrophoresis , genetics , genome , restriction enzyme , restriction map , moraxella , genome size , gene , streptococcus pneumoniae , genotype , plasmid , bacteria , anatomy
Using pulsed field gel electrophoresis (PFGE) and Southern hybridization techniques, a physical map of Moraxella catarrhalis strain ATCC25238 was constructed to provide basic genetic knowledge of this bacterium that has attracted attention in recent years as a human pathogen. Restriction endonuclease Not I cut the genome into 10 fragments and Sma I into 9, and the molecular size of the genome was estimated to be 1,940 kilobases. Location of the 12 genes participating in the biosynthesis of purine, pyrimidine and nine kinds of amino acids were determined on the circular physical map of the strain.