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Detection of Astroviruses from Stool Samples in Japan Using Reverse Transcription and Polymerase Chain Reaction Amplification
Author(s) -
Saito Kunihiro,
Ushijima Hiroshi,
Nishio Osamu,
Oseto Mituaki,
Motohiro Hiroshi,
Ueda Yuichi,
Takagi Michio,
Nakaya Shigekazu,
Ando Tamie,
Glass Rodger,
Zaiman Kohji
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03264.x
Subject(s) - serotype , biology , polymerase chain reaction , reverse transcription polymerase chain reaction , virology , reverse transcriptase , immunoassay , monoclonal antibody , astrovirus , microbiology and biotechnology , antibody , virus , genetics , gene , messenger rna , rotavirus
We developed a reverse transcription and polymerase chain reaction (RT‐PCR) method for detecting astrovirus serotypes 1, 2, 3, 5, 6 and 7 (but not serotype 4). Furthermore, we developed the specific primers for detecting serotypes 1 and 2, the most predominant serotypes in the world. Sensitivity of the first PCR with serotype common primers was about 10 times higher than that of enzyme immunoassay with monoclonal antibody (EIA‐MAb). Sensitivity of the second PCR with the serotype‐specific primers was even higher. The RT‐PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2.