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Inhibition by Bacterial Lipopolysaccharide of Spontaneous and TNF‐α‐Induced Human Neutrophil Apoptosis In Vitro
Author(s) -
Hachiya Osamu,
Takeda Yuji,
Miyata Hiroaki,
Watanabe Hiroshi,
Yamashita Takao,
Sendo Fujiro
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03247.x
Subject(s) - apoptosis , cycloheximide , tumor necrosis factor alpha , lipopolysaccharide , biology , in vitro , flow cytometry , microbiology and biotechnology , agarose gel electrophoresis , immunology , protein biosynthesis , dna , biochemistry
In the previous paper (Takeda et al, Int. Immunol., 5, 691‐694, 1993), we demonstrated that tumor necrosis factor‐α (TNF‐α) promptly accelerates apoptosis of human neutrophils in vitro . In order to determine the role of neutrophil apoptosis in defending against bacterial infection, we studied the effect of bacterial lipopolysaccharide (LPS) on this process. LPS inhibited spontaneous and TNF‐α‐induced human neutrophil apoptosis in vitro , as determined by 1) light and electron microscopy, 2) flow cytometry, and 3) agarose gel electrophoresis of DNA. Low concentrations of cycloheximide, a protein synthesis inhibitor, which alone did not affect neutrophil apoptosis, were able to reduce spontaneous apoptosis inhibition by LPS, suggesting the involvement of newly synthesized protein in this phenomenon.