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Suppression of Cytokine Production in T Helper Type 2 Cells by Nitric Oxide in Comparison with T Helper Type 1 Cells
Author(s) -
Nukaya Ikuei,
Takagi Kuniaki,
Kawabe Takefumi,
Suketa Yasunobu
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb03246.x
Subject(s) - clone (java method) , biology , omega n methylarginine , cytokine , nitric oxide , sodium nitroprusside , dna synthesis , microbiology and biotechnology , monoclonal antibody , interferon gamma , cell culture , dna , nitric oxide synthase , immunology , antibody , biochemistry , endocrinology , genetics
We examined the effect of nitric oxide (NO) on cytokine production in T helper (Th) cell subsets, using murine splenic CD4 + T cells and two types of Th clones. Interferon‐gamma‐treated murine peritoneal exudate cells (IFN‐PEC) suppressed DNA synthesis to 60% of the control level in CD4 + T cells stimulated with the anti‐CD3 monoclonal antibody. The production of IL‐2 and IL‐4 in the CD4 + T cells decreased to 63.2% and 9.2%, respectively, of the control value by co‐culture with IFN‐PEC. The addition of N G ‐monomethyl‐ L ‐arginine (L‐NMMA) partially recovered the suppression of DNA synthesis. In the presence of indomethacin, the suppression of DNA synthesis was partially inhibited and the reduction in the cytokine production caused by IFN‐PEC was partially recovered. The simultaneous addition of N G ‐monomethyl‐ L ‐arginine (L‐NMMA) and indomethacin completely inhibited not only the suppression of DNA synthesis but also the reduction in the cytokine production caused by IFN‐PEC. Moreover, DNA synthesis in the Th2 clone was suppressed to a greater extent than that in the Th1 clone by co‐culture with IFN‐PEC. This suppression in the Th1 clone was inhibited by the addition of L‐NMMA, whereas the DNA synthesis in the Th2 clone was not recovered by L‐NMMA. In addition, sodium nitroprusside (SNP) suppressed IL‐4 production in the Th2 clone but had no effect on IL‐2 production in the Th1 clone. In the experiment of the co‐culture with IFN‐PEC, the inhibitory‐effect of NO on T cell activation was not clarified by the influence of prostaglandins. However, in conclusion, cytokine production in Th2 cells may be more susceptible to NO than that in Th1 cells.