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Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis
Author(s) -
Matsuyama Junko,
Yamamoto Koichiro,
Miwatani Toshio,
Honda Takeshi
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02251.x
Subject(s) - hemolysin , monoclonal antibody , bacillus cereus , biology , microbiology and biotechnology , toxin , antibody , biochemistry , bacteria , immunology , virulence , genetics , gene
A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt‐hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt‐hemolysin but also to a hemolysin (Bc‐hemolysin) produced by B. cereus , suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt‐ and Bc‐hemolysins by immunoaffinity column chromatography. The partial N‐terminal amino acid sequence of the purified hemolysins was determined to be Ile‐Glu‐Gln‐Thr.