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A Flow Cytometric Assay Reveals a Suppression of Phagocytosis by Rabbit Defensin NP‐3A in Mouse Peritoneal Macrophages
Author(s) -
Ichinose Mitsuyuki,
Sawada Masashi
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02214.x
Subject(s) - phagocytosis , fluorescein isothiocyanate , flow cytometry , biology , microbiology and biotechnology , macrophage , defensin , peritoneal cavity , fluorescein , immunology , biochemistry , in vitro , anatomy , fluorescence , physics , antimicrobial , quantum mechanics
Phagocytosis of fluorescein isothiocyanate (FITC)‐labeled polystyrene microparticles by peritonea] macrophages from thioglycollate‐elicited mice was examined by means of flow cytometry (FCM). This assay revealed that rabbit defensin NP‐3A suppressed the phagocytosis in a dose‐dependent manner. The present results suggest that NP‐3A released from neutrophils is one of the mediators which modulates the activity of macrophages in response to infection.