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Isolation of Influenza A and B Viruses in HeLa Cells
Author(s) -
Yamanaka Takaya,
Shirasawa Hiroshi,
Yoshizawa Shigekatsu
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02213.x
Subject(s) - hela , throat , virology , biology , virus , cell culture , isolation (microbiology) , influenzavirus b , orthomyxoviridae , microbiology and biotechnology , influenza a virus , anatomy , genetics
The HeLa cell line which is one of the most popular cell lines was shown to be suitable for isolation of types A (H 3 N 2 ) and B influenza viruses from throat washings of patients. Sixty‐nine and 67 out of 147 throat washings taken from patients during the period from January to April, 1994, were positive for influenza A virus in HeLa cells and MDCK cells, respectively. Seven out of 10 throat washings taken between January and March, 1993, were positive for influenza B virus in MDCK. Of these 7, 4 were also positive for HeLa cells.