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Heat Shock Proteins in the Human Periodontal Disease Process
Author(s) -
Ando Tomohiro,
Kato Tetsuo,
Ishihara Kazuyuki,
Ogiuchi Hideki,
Okuda Katsuji
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02208.x
Subject(s) - eikenella corrodens , prevotella intermedia , actinobacillus , microbiology and biotechnology , fusobacterium nucleatum , biology , monoclonal antibody , periodontitis , heat shock protein , bacteroidaceae , bacteroides , porphyromonas gingivalis , bacteria , antibody , immunology , medicine , biochemistry , genetics , gene
The production of HSP by periodontopathic Gram‐negative bacteria was examined by SDS‐PAGE, two dimensional gel electrophoresis, and Western blotting using monoclonal antibodies against HSPs. Strains of Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Prevotella intermedia, Prevotella nigrescens, Prevotella melaninogenica , and Treponema socranskii species produced HSP which reacted with anti‐ Yersinia enterocolitica HSP 60 and/or mycobacterial 65‐kDa HSP monoclonal antibodies. It was found that gingival homogenate samples from patients with adult periodontitis reacted with anti‐human HSP 60 and bovine brain HSP 70 monoclonal antibodies. Antibodies which reacted with bacterial HSP were also found in a serum sample from a periodontitis patient. The present study suggests that HSPs are implicated in the human periodontal disease process.