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Natural Killer (NK)‐Like Cytotoxicity of Murine Intraepithelial Lymphocytes in the Small Intestine (iIEL) and the Effect of the Serine Proteases
Author(s) -
Kato Yutaka,
Yokochi Takashi,
Maeda Kenji,
Sasaki Keiko,
Kawamoto Yasuko,
Tsuji Takao,
Miyama Akio
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02204.x
Subject(s) - cytotoxicity , proteases , intraepithelial lymphocyte , biology , serine protease , serine , flow cytometry , microbiology and biotechnology , protease , biochemistry , immunology , in vitro , enzyme , immune system
The natural killer (NK)‐like cytotoxicity of murine intraepithelial lymphocytes in the small intestine (iIEL) and the participation of serine proteases in it were investigated. We monitored the cytotoxicity of iIEL with a sensitive cytotoxic assay using laser flow cytometry. iIEL exhibited NK‐like cytotoxicity on YAC‐1 target cells. Benzamidine, a serine protease inhibitor, inhibited significantly both Na‐CBZ‐ l ‐lysine thiobenzyl ester (BLT)‐specific serine protease activity and iIEL‐mediated NK‐like cytotoxicity. These results suggest that BLT‐specific serine proteases may participate in NK‐like cytotoxicity of murine iIEL.