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Frequent Detection of Hepatitis C Virus Subtype 3a (HCV‐3a) Isolates in Thailand by PCR Using Subtype‐Specific Primers
Author(s) -
Apichartpiyakul Chatchawann,
Miyajima Hirofumi,
Doi Hisaya,
Mizokami Masashi,
Homma Morio,
Hotta Hak
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02203.x
Subject(s) - virology , hepatitis c virus , biology , serotype , polymerase chain reaction , virus , flaviviridae , viral disease , recombinant dna , hepacivirus , hepatitis c , gene , genetics
By means of a polymerase chain reaction (PCR) method using subtype‐specific primers for hepatitis C virus (HCV) subtypes 1a, 1b, 2a, 2b and 3a, the prevalence of each subtype among HCV isolates in Chiang Mai, Thailand, was determined. HCV‐3a appeared to be the most common subtype in blood donors, and was also frequently found in patients with liver disease. HCV‐1b, but not HCV‐2a or −2b, was also commonly found in this area, while a considerable percentage of the total HCV isolates still remained unclassifiable by the above methods. Serotype analysis of the HCV isolates using C14‐1 and C14‐2 recombinant peptides revealed that HCV‐3a was likely to carry an antigenic determinant(s) different from those of the major types 1 (HCV‐1a and −1b) and 2 (HCV‐2a and −2b).