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Differentiation of Clostridium difficile, Clostridium bifermentans, Clostridium sordellii , and Clostridium perfringens from Diarrheal Stool by API ZYM and API LRA Oxidase Test
Author(s) -
Fontana Carla,
Jezzi Teresa,
Testore Gian Piero,
Dainelli Benedetto
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02194.x
Subject(s) - microbiology and biotechnology , clostridium difficile , clostridiaceae , clostridium perfringens , biology , clostridia , clostridium , agar , clostridiales , bacteria , toxin , genetics , antibiotics
A simple, rapid and reliable outline for identification of clostridia isolates from human infections was developed. It consists of a combination of API ZYM and API LRA Oxidase tests. The enzymatic activities were performed with strains sub‐cultured onto carbohydrate‐free medium (Columbia blood agar). Fifty‐five strains of Clostridium difficile, C. bifermentans, C. sordellii , and C. perfringens from clinical specimens and eight reference standard strains representing different species of the same genus were analyzed. The accuracy of the new method was evaluated by comparison with the results obtained by DNA/DNA analysis.