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Inhibition of Varicella‐Zoster Virus Glycoprotein Expression by Peripheral Blood Mononuclear Cells
Author(s) -
Mizutani Kenichi,
Ito Masahiro,
Kamiya Hitoshi,
Sakurai Minoru
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02192.x
Subject(s) - peripheral blood mononuclear cell , varicella zoster virus , biology , virology , virus , flow cytometry , tumor necrosis factor alpha , microbiology and biotechnology , glycoprotein , immunology , in vitro , biochemistry
The effect of peripheral blood mononuclear cells (PBMC) on expression of varicella‐zoster virus (VZV) glycoproteins (Gps) was analyzed by flow cytometry. PBMC from VZV seropositive and seronegative donors and supernatant of PBMC co‐cultured with VZV‐infected human embryonic fibroblasts reduced VZV Gp expression. Neutralization of supernatant fluid with mixture of anti‐interferons (IFN)‐α, ‐β, ‐γ, and tumor necrosis factor (TNF)‐α partially reduced inhibitory activity of supernatant on VZV Gp expression. Deletion of natural killer (NK) cells and adherent cells from PBMC reduced inhibitory activity of PBMC on VZV Gp expression. These results suggest that IFN‐α, ‐β, ‐γ, TNF‐α and other soluble factors released from NK cells and monocytes by co‐cultivation with VZV‐infected fibroblasts inhibit VZV Gp expression.