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In Vivo Production of Various Cytokines in Splenocytes of Sheep Erythrocyte‐Immunized Mice after Intravenous Administration of Bacterial Lipid A
Author(s) -
Mashimo Junichi,
Mita Akira
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02185.x
Subject(s) - lipopolysaccharide , titer , immunization , biology , in vivo , lipid a , antigen , antibody , immunology , splenocyte , adjuvant , antibody titer , microbiology and biotechnology
The effects of an intravenous administration of lipid A from Salmonella minnesota R595 lipopolysaccharide on the in vivo production of interleukin‐2 (IL‐2), IL‐4, IL‐5 and IL‐6 in the spleens of mice intravenously immunized with sheep red blood cell (SRBC) antigen were investigated. The increased number of antigen‐specific IgM antibody‐producing cells and the titer of the IgM serum antibody were measured using the plaque‐forming cell (PFC) assay and an enzyme‐linked immunosorbent assay (ELISA). Simultaneous injections of SRBC antigen and lipid A adjuvant enhanced IgM‐PFC number on days 3 and 4 and the serum IgM titer on days 4 and 5 after the immunization. We found that the enhanced IL‐4 and IL‐5 levels correlated with the PFC number and IgM titer. When lipid A was injected intravenously 2 days after immunization with SRBC, the PFC number in lipid A‐treated groups were similar to those in controls 3 and 4 days after the immunization. However, it was found that a twofold increase in the IgM titer in serum was induced by lipid A 5 days after immunization. In relation to this increase, lipid A stimulated the production of only IL‐5 among the cytokines tested.