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Increased Expression of Adhesion Molecules (CD54, CD29 and CD44) on Fibroblasts Infected with Cytomegalovirus
Author(s) -
Ito Masahiro,
Watanabe Masahiro,
Ihara Toshiaki,
Kamiya Hitoshi,
Sakurai Minoru
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02179.x
Subject(s) - biology , cell adhesion molecule , cd44 , virology , flow cytometry , virus , herpes simplex virus , microbiology and biotechnology , cytomegalovirus , immunology , herpesviridae , in vitro , viral disease , biochemistry
The expression of ICAM‐1 (CD54), β1 integrin (CD29), and CD44 on cytomegalovirus (CMV)‐infected human embryonic fibroblasts (HEF) was analyzed by flow cytometry. The expression of these adhesion molecules increased significantly on CMV‐infected HEF, on days 2 and 5 after inoculation, compared to uninfected HEF. However, the expression of these adhesion molecules decreased on herpes simplex virus (HSV)‐1 and varicella‐zoster virus (VZV)‐infected HEF. Increased expression was not observed on HEF treated either with inactivated CMV or with supernatant fluid of CMV‐infected cells. The addition of anti‐cytokine (TNF‐α, IL‐1β, or IFN‐γ) antibodies had no effect on the increase of these adhesion molecules. This suggests that the increase in CD54, CD29, and CD44 on CMV‐infected cells requires active virus replication and was not mediated by a soluble factor released from CMV‐infected cells. Changes in adhesion molecules on CMV‐infected fibroblasts may contribute to inflammation induced by CMV infection.