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Carbohydrate‐Dependent Biological Activities of Glycosylated Human Interferon‐β on Human Hepatoblastoma Cells In Vitro
Author(s) -
Utsumi Jun,
MatsuoOgawa Etsuko,
Nagahata Takemitsu,
Kasama Kyoko,
Kagawa Yasuhiro,
Satoh Yuichiro
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02173.x
Subject(s) - chinese hamster ovary cell , biology , hepatoblastoma , recombinant dna , interferon , in vitro , biological activity , microbiology and biotechnology , interferon alfa , cell culture , alpha interferon , virology , biochemistry , gene , medicine , genetics
To evaluate the relationship between the sugar chain structure and biological activity, fibroblast‐derived glycosylated human interferon‐β, Chinese hamster ovary cell‐derived glycosylated recombinant human interferon‐β and Escherichia coli ‐derived unglycosylated recombinant human interferon‐β were evaluated using human hepatoblastoma cells in vitro . Native fibroblast interferon‐β expressed more cell‐growth inhibitory action, 2′5′‐oligoadenylate synthetase induction, and the inhibition of hepatitis B virus DNA replication than its asialoform and two recombinant interferon‐βs. These results showed that the sugar chain structure of human interferon‐β affects its biological activity on human hepatoblastoma cells.