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Production of Antigenically Related Exocellular Elastolytic Proteases Mediating Hemagglutination by Vibrios
Author(s) -
Alam Munirul,
Miyoshi Shinichi,
Shinoda Sumio
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02170.x
Subject(s) - proteases , protease , biology , microbiology and biotechnology , virulence , metalloproteinase , hemagglutination , vibrio vulnificus , vibrio , antigen , enzyme , virology , bacteria , biochemistry , immunology , gene , genetics
Exocellular proteases produced by Vibrio fluvialis, V. furnissii, V. metschnikovii and V. campbellii were characterized and compared to those of V. mimicus protease (VMP) and V. vulnificus protease (VVP). These proteases possessed both elastolytic and hemagglutinating abilities and were identified, except that of V. metschnikovii , as metalloprotease. Conversely, V. metschnikovii protease failed to exhibit some of the salient features for metalloproteases suggesting the existence of protease(s) other than metalloprotease. However, antibodies against VVP cross‐reacted to these proteases and to VMP indicating antigenic relatedness amongst vibrio proteases. This study, thus, demonstrated the prevalent distributions of antigenically related proteases both in pathogenic and non‐pathogenic vibrios, bringing their status as a virulence determinant into question.