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Epitope Analysis Using Anti‐Oligosaccharide (G4) Monoclonal Antibody
Author(s) -
Hirata Akio,
Komoda Makiko,
Itoh Wataru,
Tabata Kengo,
Sugawara Isamu
Publication year - 1995
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1995.tb02166.x
Subject(s) - monoclonal antibody , epitope , microbiology and biotechnology , antibody , biology , conjugate , splenocyte , monoclonal , polysaccharide , antigen , antigenicity , virology , biochemistry , immunology , mathematical analysis , mathematics
A murine monoclonal antibody recognizing (1→6)‐β‐ d ‐glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4‐bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyllan, an antitumor polysaccharide, but notable reactivity with some low‐molecular‐weight schizophyllans. This antibody was useful for determination of the epitope of several polysaccharides. The extent of reactivity of this monoclonal antibody was related only to the molecular weight of schizophyllan.