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Thy.1 low CD3 − Cells Sorted from Nylon Wool‐Passed Bone Marrow Cells Can Augment the H‐2 Identical but Not Non‐Identical Cytotoxic T Lymphocyte Precursors in Mixed Lymphocyte Cultures
Author(s) -
Mukouyama Hideki,
Tanabe Masao J.
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb02153.x
Subject(s) - cytotoxic t cell , ctl* , microbiology and biotechnology , biology , cd8 , bone marrow , antigen presenting cell , spleen , major histocompatibility complex , t cell , antigen , immunology , immune system , biochemistry , in vitro
Thy. 1 low CD3 – cells obtained from nylon wool‐passed murine bone marrow (NW‐BM) cells by cell sorting did not express CD4, CD8, or T cell receptor‐ α/β and ‐γ/δ on their cell surfaces. An extremely limited number of B10.BR (H‐2 k ) responder lymph node (LN) cells were stimulated with B10. D2 (H‐2 d ) stimulator spleen cells in cultures containing the minimum required dose of rat T cell growth factor (TCGF). In these cultures, the generation of cytotoxic T lymphocytes (CTL) was very low. B10.BR Thy.1 low CD3 – NW‐BM cells, added to these cultures, could augment the CTL generation vigorously, but neither B10 (H‐2 b ) nor B10.D2 cells could. When B10 LN cells were used as responder cells in these cultures, B10 Thy. 1 low CD3 – NW‐BM cells could augment the CTL generation, but neither B10.BR nor B10.D2 cells could. Similar findings were obtained when Lyt‐2 + cells or Thy.1 + L3T4 – (CTL precursor) cells sorted from spleen cells were used as responder cells. Both elements, rat‐TCGF and Thy.1 low CD3 – NW‐BM cells, were essential for this augmentation of the CTL generation in this culture system because neither one alone could augment generation, and rat‐TCGF could be replaced by Thy.1 + Lyt‐2 – helper T (Th) cells sorted from spleen cells. These findings showed that NW‐BM cells could augment CTL precursors in a self‐major histocompatibility complex (self‐MHC)‐antigen restricted manner, and further that both NW‐BM cells and Th cells had different and independent functions to induce CTL.