Premium
Alteration of Immune Responses of Rabbits Infected with Bovine Immunodeficiency‐Like Virus
Author(s) -
Hirai Nobuaki,
Xuan Shiwei,
Ochiai Kenji,
Onuma Misao
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb02151.x
Subject(s) - biology , spleen , immune system , antibody , peripheral blood mononuclear cell , virology , virus , lymphatic system , lymph , bovine serum albumin , immunology , humoral immunity , in vitro , medicine , pathology , biochemistry
Nine 3‐month‐old rabbits were inoculated with bovine immunodeficiency‐like virus (BIV) to study the pathogenesis of BIV and alteration of the immune responses in experimentally infected rabbits. BIV proviral DNA and anti‐BIV antibodies were detected from all rabbits inoculated with BIV‐infected bovine embryo spleen (BESP) cells. Rabbits inoculated with spleen cells of the BIV‐infected rabbit also converted to proviral DNA‐positive and BIV‐antibody‐positive. The blastogenic responses to concanavalin A of peripheral blood mononuclear cells prepared from BIV‐infected rabbits were not significantly different from those from uninfected controls at 2 and 4 months post‐inoculation (PI). The humoral immune responses against bovine serum albumin (BSA) were depressed in two of four BIV‐infected rabbits at 1 to 3 months PI. The antibody responses against sheep red blood cells (SRBCs) were significantly depressed in all BIV‐infected rabbits at 2 to 4 months PI. BIV was rescued by cocultivation of spleen cells of infected rabbits with BESP cells. Distinct development of lymphoid follicle was observed in lymph nodes and spleens of uninfected rabbits which received BSA and SRBCs. In contrast, moderate lymphoid cell depletion was observed in BIV‐infected rabbits which received the same immunogens.