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Inhibition of T‐Cell Proliferation Induced by a Cell‐Free Salmonella typhimurium Extract Does Not Involve a Nitric Oxide‐Mediated Mechanism
Author(s) -
Matsui Katsuhiko,
Arai Toshihiko
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb02147.x
Subject(s) - biology , nitric oxide , cell growth , t cell , interferon gamma , interleukin 2 , salmonella , cell , monoclonal antibody , spleen , cell culture , microbiology and biotechnology , cytokine , immunology , antibody , immune system , endocrinology , biochemistry , bacteria , genetics
In a previous study, we observed that a cell‐free Salmonella typhimurium extract induced suppression of mitogen‐induced T‐cell proliferation and that this suppression involved non‐responsiveness of T‐cells to interleukin‐2 (IL‐2) and augmentation of IL‐2 receptor (IL‐2R) expression. In this study, we found that inhibition of phytohemagglutinin (PHA)‐stimulated murine spleen cell proliferation induced by a cell‐free S. typhimurium extract was reversed by treatment with an anti‐interferon‐γ monoclonal antibody (anti‐IFN‐γ Ab), but not by interleukin‐4 or N G ‐monomethyl‐ l ‐arginine, which is known to inhibit nitric oxide (NO)‐secretion from spleen cells in culture. However, IL‐2R expression was augmented by treatment with the extract, although this was independent of an NO‐mediated mechanism. Only anti‐IFN‐γ Ab treatment reduced the augmented IL‐2R expression to a normal level. These results suggest that the suppression of T‐cell proliferation induced by the Salmonella cell‐free extract is associated with augmentation of IL‐2R expression in an NO production‐independent manner.