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Increased Phosphodiesters in Lipopolysaccharide Prepared from the Polymyxin B‐Resistant Isolate of Klebsiella pneumoniae
Author(s) -
Yokochi Takashi,
Inoue Yoshiko,
Jiang Guozhi,
Kato Yutaka,
Sugiyama Tsuyoshi,
Kawai Makoto,
Fukada Masako,
Takahashi Kazuko
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb02144.x
Subject(s) - biology , mutant , polymyxin b , klebsiella pneumoniae , polymyxin , microbiology and biotechnology , lipopolysaccharide , phosphomonoesters , biochemistry , phosphate , immunology , antibiotics , escherichia coli , gene , inorganic phosphate
A polymyxin B (PXB)‐resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB‐resistant isolate bound little PXB, although LPS from the parental strain did. The 31 P nuclear magnetic resonance (NMR) spectrum of PXB‐resistant type LPS showed that it contained much less of the phosphomonoesters and the pyrophosphate esters, and an increased amount of the phosphodiesters, compared to the parental type LPS. The decrease in the binding of PXB might be due to altered phosphate groups on the PXB‐resistant type LPS, suggesting that it might explain the PXB‐resistance of the mutant.