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Growth Medium Containing Cyclodextrin and Low Concentration of Horse Serum for Cultivation of Helicobacter pylori
Author(s) -
Morshed Muhammad Golam,
Karita Mikio,
Konishi Hisanori,
Okita Kiwamu,
Nakazawa Teruko
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb02143.x
Subject(s) - helicobacter pylori , urease , brucella , biology , horse , microbiology and biotechnology , bacteria , microaerophile , brucella abortus , bacterial growth , cyclodextrin , isolation (microbiology) , food science , biochemistry , immunology , enzyme , brucellosis , paleontology , genetics
The growth of Helicobacter pylori , a Gram‐negative microaerophilic bacterium, is often difficult and requires complex media with the supplementation of 5% to 10% blood or blood derivatives. We have found that Brucella broth supplemented with 1% heated horse serum and 0.1% β‐cyclodextrin supports the good growth of H. pylori . The degree of growth and production of urease and vacuolating cytotoxin in this medium were equal to those in the medium supplemented with 5% horse serum. This medium was found to be suitable for both the routine laboratory culture and primary isolation of H. pylori from biopsy samples.