Premium
Lipofection of Synthetic Oligodeoxyribonucleotide Having a Palindromic Sequence of AACGTT to Murine Splenocytes Enhances Interferon Production and Natural Killer Activity
Author(s) -
Yamamoto Toshiko,
Yamamoto Saburo,
Kataoka Tetsuro,
Tokunaga Tohru
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01867.x
Subject(s) - splenocyte , biology , palindromic sequence , palindrome , interferon , interferon gamma , microbiology and biotechnology , spleen , natural killer cell , receptor , cell culture , immunology , virology , cytokine , in vitro , biochemistry , cytotoxicity , gene , genetics , crispr
A synthetic 22‐mer oligodeoxyribonucleotide having an AACGTT palindrome, AAC‐22, induced interferon (IFN) production and augmented the natural killer (NK) activity in murine splenocytes, whereas its analogue, ACC‐22, having an ACCGGT palindrome, did not. The binding of AAC‐22 to splenocytes was not different from that of ACC‐22. Lipofection of AAC‐22 to splenocytes remarkably enhanced IFN production and NK cell activity, whereas that of ACC‐22 caused little enhancement. These results strongly suggest that the prerequisite for IFN production is not the binding of AAC‐22 to the cell surface receptors, but its penetration into the spleen cells.