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A Novel Assay System for Anti‐Human Immunodeficiency Virus Type 1 (HIV‐1) Activity Using a Subclone of a Monocytic Cell Line, U937
Author(s) -
MiyanoKurosaki Naoko,
Nakashima Hideki,
Ichiyama Koji,
Inazawa Kazuhiko,
Tabata Hidenori,
Tanabe Hideyuki,
Ohnishi Kiyotaka,
Mizusawa Hiroshi,
Ohshiro Yukako,
Yamamoto Naoki
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01863.x
Subject(s) - u937 cell , biology , cell culture , virology , microbiology and biotechnology , virus , ec50 , monocyte , in vitro , immunology , biochemistry , genetics
A subclonal cl.1–14 cell was established from a monocytic cell line U937 by a limiting dilution method. The anti‐HIV‐1 activity of some antiviral compounds was evaluated in HIV‐1‐infected cl.1–14 cells. The results demonstrated that although AZT was a potent inhibitor of HIV‐1 replication in cl.1–14 cells, its 50% effective concentration (EC 50 ) values was 80 times higher than that in HIV‐1 infected MT‐4 cells; the EC 50 of AZT was 0.16 μM and 0.002 μM in cl.1–14 and MT‐4 cells, respectively. In contrast, the anti‐HIV‐1 activity of ddA, ddI and ddC in cl.1–14 cells was comparable to that in MT‐4 cells. The antiviral activity of nevirapine, dextran sulfate, curdlan sulfate and T22 did not differ significantly between the cl. 1–14 and MT‐4 cells. The antiviral activity of several compounds in the HIV‐1‐infected cl.1–14 cells was similar to that in the HIV‐1 jr ‐ fl ‐infected human peripheral macrophages. Our results suggest that cl.1–14 cell cultures are very useful for estimating antiviral activity and more advantageous than the use of peripheral blood macrophages.