Premium
Detection of Human Cytomegalovirus DNA in Breast Milk by Means of Polymerase Chain Reaction
Author(s) -
Hotsubo Tomoyuki,
Nagata Nobuo,
Shimada Masayoshi,
Yoshida Koichi,
Fujinaga Kei,
Chiba Shunzo
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01862.x
Subject(s) - human cytomegalovirus , polymerase chain reaction , breast milk , biology , cytomegalovirus , virology , human breast milk , betaherpesvirinae , virus , herpesviridae , viral disease , genetics , gene , biochemistry
Three hundred and twenty‐five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.