Premium
Complement‐Mediated Killing of Borrelia garinii —Bactericidal Activity of Wild Deer Serum
Author(s) -
Isogai Emiko,
Kamewaka Yuri,
Isogai Hiroshi,
Kimura Koichi,
Fujii Nobuhiro,
Nishikawa Takeshi
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01852.x
Subject(s) - borrelia garinii , borrelia , biology , microbiology and biotechnology , lyme disease , in vitro , antibody , strain (injury) , borrelia burgdorferi , virology , immunology , biochemistry , anatomy
The susceptibility of Borrelia garinii to fresh wild deer sera was determined by incubating strain SIKA2 at 10% serum concentration for 1 hr at 37 C in an in vitro bactericidal assay. Each serum showed bactericidal effects at various levels. The effect was dependent on the concentration of antibody to the spirochetes. Complement was essential in the bactericidal assay because the inactivated deer serum showed greatly decreased activity. Our results suggest that B. garinii is sensitive to deer serum, in the presence of antibody and the bactericidal effect is important for preventing Lyme disease in wild sika deer.