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Detection of DNA of Causative Agent of Spotted Fever Group Rickettsiosis in Japan from the Patient's Blood Sample by Polymerase Chain Reaction
Author(s) -
Tange Yoshiki,
Matsumoto Mitsuru,
Okada Takanori,
Hasegawa Hitoshi,
Kobayashi Yuzuru
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01837.x
Subject(s) - rickettsiosis , polymerase chain reaction , biology , rickettsia rickettsii , spotted fever , virology , rickettsia , q fever , gene , genetics , virus
The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17‐kDa antigen gene of Rickettsia rickettsii primed a rickettsia‐specific 246‐base‐pair product for all of the Katayama, Abe, Misaka and Kojima strains, which we had isolated previously. Moreover, we were able to detect the same product by PCR amplification from the peripheral blood of a patient in the acute stage of the illness. The PCR method is considered to be useful for rapid etiological diagnosis of SFG rickettsiosis in Japan.