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Crystallization and Analyses of Crystals of Various Chemotypes of R‐Form Lipopolysaccharides from Salmonella Spp.
Author(s) -
Kato Nobuo,
Arakawa Yoshichika,
Sugiyama Tsuyoshi,
Ito Hideo,
Naito Setsuko,
Kido Nobuo,
Ohta Michio,
Sasaki Kyoyu
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01833.x
Subject(s) - crystallography , diffraction , electron diffraction , crystallization , x ray crystallography , materials science , chemistry , physics , optics , organic chemistry
Various chemotypes (Re, Rd 2 , Rd 1 P – , Rd 1 , RcP – , Rc, Rb 3 , Rb 2 , Rb 1 , and Ra) of R‐form lipopolysaccharides (LPSs) of Salmonella spp. were crystallized by treatment with 70% ethanol containing 250 mM MgCl 2 , and crystals of the LPSs were observed electron microscopically and analyzed by electron diffraction and synchrotron X‐ray diffraction. All the LPSs tested formed three‐dimensional crystals showing very similar shapes; hexagonal plate, solid column, discoid, square or rectangular plate, lozenge plate and truncated hexangular or rectangular pyramid forms. Electron diffraction patterns from the hexagonal plate crystals of all these LPSs obtained by electron irradiation from the direction perpendicular to the basal plane showed that they consist of hexagonal lattices with the lattice constant of 4.62 Å. The crystals of all the LPSs thus formed gave ring‐like X‐ray diffraction patterns because of their small sizes. The long‐axis values were calculated from the X‐ray diffraction patterns from crystals of all the LPSs in the low‐angle region and they corresponded roughly to the length of the proposed primary chemical structures of the R cores of the LPSs. The volume occupied by a single molecule of all the LPSs were calculated from the molecular weights based on the proposed structures and the crystallographic data obtained by electron diffraction, X‐ray diffraction, and density determination.